Fig 1: METTL3 activated calcineurin/NFAT signaling via promoting TRPC6. rPAECs were divided into the following groups: Nx, Hx, Hx + lv-shNC, Hx + lv-shMETTL3, Hx + HF, and Hx + lv-shMETTL3 + HF. After indicated treatment, the subsequent experiments were conducted. (a) Western blot assessed the protein level of TRPC6. (b) The changes in calcineurin activity were detected by using the commercial kit. (c) The transcriptional activity of NFAT was detected by the luciferase assay. (d) Western blot quantified the levels of NFATc1, NFATc2, NFATc3, and NFATc4. *P < 0.05, **P < 0.01, and ***P < 0.001.